A study of the biology, methodology, and applications of in vitro cultures of animal cells. This course explores the theoretical bases for the in vitro isolation, maintenance, propagation and identification of living cells as well as practical experience with common cell and tissue culture techniques. Topics include laboratory safety and equipment; primary and established cell lines; anchorage dependence; the biology of cultured cells; cell culture environments; contamination; cell harvesting and separation; cell transformation, differentiation, cloning, genetic engineering, and stem cells. Lectures and laboratories. Prerequisites: Biology 224 (or 141); Chemistry 253 (or 261 and 262).