BIOL-334   Cell and Tissue Culture

Ever wonder what's required for animal cells to live and reproduce outside a multicellular organism? Do they continue their specialized functions? Can they live forever? This course explores the biology, methodology, and applications of animal cell culture, likely the most commonplace and fastest growing technology for studying mammalian cells and harvesting their products. Topics include primary and established cell lines, anchorage dependence, culture environments, including two- and three-dimensional systems, contamination, bioreactors, transformation, immortalization, differentiation, cloning, genetic engineering, and stem cells. Lectures and laboratories. Prerequisites: Biology 224 (or 141), Chemistry 253 (or 261 and 262). Not offered 2013-2014.